The researchers then investigated how different gene expressions in the cells changed over time. They used different concentrations of the vaccine and The results have in many cases been misinterpreted, the Q&A also stated. official website and that any information you provide is encrypted :\rBuy me a coffee :-) https://www.buymeacoffee.com/DrMobeenSyed\rBecome my patron: https://www.patreon.com/mobeensyed?fan_landing=true\rPayPal: https://paypal.me/mobeensyed?locale.x=en_US\r\r#drbeen #koolbeens #COVID\r\rChapters00:00 introduction00:45 Link to the summary01:00 Review of the reference material02:19 Cell preparation for the vaccine03:35 Vaccine addition to the cells06:00 RT-PCR for find RNA and then DNA13:39 Imaging of the cells and the LINE-1 protein, ORF-1 protein, and the DNA18:27 Measuring the volume of proteins in the cytosol and the nucleus22:35 DNA purification and verification28:41 Results28:42 LNP enters the liver cells efficiently29:33 LINE-1 gene is expressed30:46 ORF-1 found in cytosol and the nucleus36:01 DNA from the vaccine found in the cell43:30 Questions not answeredDisclaimer:\rThis video is not intended to provide assessment, diagnosis, treatment, or medical advice; it also does not constitute provision of healthcare services. It is important to bear in mind that the liver cells in this study are more genetically unstable than our own liver cells. Further studies are needed to demonstrate the effect of BNT162b2 on genomic integrity, including whole genome sequencing of cells exposed to BNT162b2, as well as tissues from human subjects who received BNT162b2 vaccination, the authors said. Getty N. Engl. These cells have had the normal protections of the immune system removed.. DNA amplicons (444 bps) were visualized on agarose gel. Shakira. In fact. Thrombotic events have been reported as a rare side effect after ChAdox1nCOV-19 vaccination that may cause death of recipient. Federal government websites often end in .gov or .mil. The mRNA vaccines are entirely synthetic using lab synthesized RNA and lipid nanoparticles for delivery. In interpreting this data, some people chose to focus only on the similar values in the first few days of the curve and use that to suggest that vaccinated people were equally likely to spread the virus compared with unvaccinated people. We show that the vaccine enters liver cells as early as six hours after the vaccine has been administered. The patient If such DNA had been incorporated into the genome, as suggested in the false claims, the fear is that it could potentially alter cell function or lead to cancer in certain cases. The Centers for Disease Control and The two that have made the news recently are the Pfizer vaccine and the Moderna vaccine. First, cancerous cell lines replicate, whereas our liver cells typically are not replicating. selenagomez. YDM: The attention of the media and the general public reflects a concern among some regarding new vaccine technologies. Whether the findings of the study will occur in living organisms or if the DNA converted from the vaccines mRNA will integrate with the cells genome is unknown. The study, conducted by researchers at Lund University in Sweden and published in a peer-reviewed journal, investigated the effect of Pfizers mRNA COVID-19 vaccine on a human cell line that was derived from liver cancer tissue. The Swedish study also found spike proteins expressed on the surface of the liver cells that researchers say may be targeted by the immune system and possibly cause autoimmune hepatitis, as there [have] been case reports on individuals who developed autoimmune hepatitis after BNT162b2 vaccination.. Childrens Hospital of Philadelphia is a charitable 501(c)(3) nonprofit organization. This is part of APs effort to address widely shared misinformation, including work with outside companies and organizations to add factual context to misleading content that is circulating online. Its May 03, 2022 Virological and serological kinetics of SARS-CoV-2 Delta variant vaccine breakthrough infections: a multicentre cohort study. and Terms of Use. Dr. Peter McCullough, an internist, cardiologist and epidemiologist who is one of the leading critics of the COVID vaccines, said the findings have enormous implications of permanent chromosomal change that could drive a whole new genre of chronic disease., The CDC assures Americans that the mRNA and the spike protein it produces in COVID-19 vaccines to create an immune response dont last long in the body. On its website, the agency states: Our cells break down mRNA and get rid of it within a few days after vaccination. PCR was run on all samples with primers targeting BNT162b2, as shown in Figure 1 and Table 1. Click here:\rhttps://www.drbeen.com/yt-special-p/\r\rWant to support this work? 8600 Rockville Pike Whether our results are true for other cell types in humans, or if they are specific to mRNA vaccines, are among many questions for further research. They have somewhere between 90 to 95% efficacy at providing immunity to someone No added sugar, full of protein and chocolate! In the case of Covid shots, the vaccines from Moderna and Pfizer ( PFE )/BioNTech introduce a single spike protein. WebPfizer Vaccine Becomes DNA in Liver Cells. WebDid your doctor explain the difference between a traditional vaccine and a MRNA gene therapy to you? We show that the vaccine enters liver cells as Five patients (42 percent) were evaluated for liver transplantation because of persistent jaundice, hepatic insufficiency, and/or recurrent cholangitis. The study tested whether the vaccines mRNA could be converted to DNA, and found that this was the case in certain lab-altered liver cell lines under experimental conditions. Parents PACK May 2022, Feature Article. Preclinical studies of COVID-19 mRNA vaccine BNT162b2, developed by Pfizer and BioNTech, showed reversible hepatic effects in animals that received the In order to create these cell lines, the genetic make-up of the cells has to be fiddled with to make them immortal and keep them alive in the petri dish, Dr. David Strain, a senior clinical lecturer at Englands University of Exeter Medical School, wrote in an email to the AP. PCR primer set used to detect mRNA level and reverse-transcription of BNT162b2. Huh7, MeSH 2020;383:26032615. Privacy policy Social media users are falsely claiming that a Swedish study conducted by Lund University found that mRNA COVID-19 vaccines permanently alter human DNA. A gene that the researchers studied produces the protein LINE-1. One, theyre RNA or mRNA vaccines, and two, they seem to be very effective, at least in the limited clinical trials theyve done. Curr Issues Mol Biol. More information: This will lead to some parts of our website to not work properly or at all. Bookshelf Our results indicate a fast up-take of BNT162b2 into human liver cell line Huh7, leading to changes in LINE-1 expression and distribution. or, by Lund University. Before To understand why this is unlikely, one needs to know a bit about protein chemistry: An analysis like the one in this paper may allow one to create an interesting hypothesis, but it does not provide evidence of a concern. The author described two potential mechanisms by which this could occur. And even if there was, what are you going to do with 147 kids' data?7 The pharmaceutical industry is dangerous to health. At the heart of the misconceptions about this paper is figure 1, which shows viral shedding among vaccinated (green) and unvaccinated (red) individuals. 2022;44(3):1115-26. The researchers found that when the mRNA vaccine enters the human liver cells, it triggers the cells DNA, which is inside the nucleus, to increase the production of the LINE-1 gene expression to make mRNA. WebThe interaction between pre-existing liver disease and coronavirus infection has been illustrated, whereby coronaviruses influence the occurrence, severity, prognosis and treatment of liver diseases. MR: We understood that the study would attract attention, but we think it is self-evident that this type of research should be pursued. For general feedback, use the public comments section below (please adhere to guidelines). In thisstudywe present evidence that COVID-19 mRNA vaccine BNT162b2 is able to enter the human liver cell line Huh7 in vitro, the researchers wrote in the study, published inCurrent Issues of Molecular Biology. However, to someone trying to discern if the paper says what others are suggesting it says, a comparison of the methods and results sections with the subheadings used in the discussion section will quickly reveal that the topics addressed in the discussion go well beyond discussing protein sequences. doi: 10.7759/cureus.32361. Please enable it to take advantage of the complete set of features! This is part of APs effort to address widely shared misinformation, including work with outside companies and organizations to add factual context to misleading content that is circulating online. A new Swedish study published in MDPIfound that the Pfizer vaccine goes into liver cells and converts to DNA, challenging claims so far that the mRNA COVID-19 vaccines do not change or interact with your DNA in any way. The views expressed in this article are those of the author and do not reflect the official position of yourNEWS. Its precisely what health experts and fact-checkers said for more than a year could not occur. Heres why: Lyons-Weiler J. Pathogenic priming likely contributes to serious and critical illness and mortality in COVID-19 via autoimmunity. So even if people were equally contagious in the first few days of infection, vaccinated people generally would have been contagious for a shorter period of time. badbunny. As such, consumers of information should always consider messages with a questioning attitude. The PubMed wordmark and PubMed logo are registered trademarks of the U.S. Department of Health and Human Services (HHS). Furthermore, a recent study showed that SARS-CoV-2 RNA can be reverse-transcribed and integrated into the genome of human cells. However, this is not an appropriate conclusion from this paper for several reasons: Logical fallacy called hasty generalization (It is important to note in this case, that the misconception was not because of the quality of the science or the messaging of the authors, but rather because others took the findings out of context.). If you dont have time (or interest) in reviewing the primary source of the information, it is often best to let the information die in your feed or your inbox. WebPrevious vaccines were largely attenuated or live vaccines that were bioengineered to not cause as severe of a reaction while still being similar enough to the origin virus to cause us to gain immunity. The experiment was conducted in a petri dish and aimed to answer the question of whether the mRNA in the vaccine could be converted to DNA. 2023 Jan 19. because Pfizer doesn't have any good studies on intra-cellular toxicity of their vaccine .. it leaves behind genetic markers that may indicate a change in DNA of the The information you enter will appear in your e-mail message and is not retained by Medical Xpress in any form. Huh7 cells were treated without (Ctrl) or with 0.5, 1, and 2 g/mL of BNT162b2 for 6 h. Cells were fixed and stained with antibodies binding to LINE-1 ORF1p (red) and DNA-specific probe Hoechst for visualization of cell nucleus (blue). Likewise, even if these data were found to be representative of ones ability to spread the virus, it is not appropriate to dismiss the rest of the curve, which shows differences between vaccinated and unvaccinated people after day four or five. Huh7 cells were treated without (Ctrl) or with 0.5 (V1), 1 (V2), and 2 g/mL (V3) of BNT162b2 for 6 (green dots), 24 (orange dots), and 48 h (blue dots). Shakira. Huang X, Kong N, Zhang X, Cao Y, Langer R, Tao W. Nat Med. The. Epub 2022 Feb 3. 2022 Nov;28(11):2273-2287. doi: 10.1038/s41591-022-02061-1. The experiment was conducted in a petri dish and aimed to answer the question of whether the mRNA in the vaccine could be converted to DNA. DNA, which makes up our genetic code, is larger, double stranded and very long. doi: 10.1503/cmaj.211881. Currently the Pfizer-Biontech (mRNA), Sinovac (inactivated virus) and Astrazeneca-Oxford (adenovirus-vector) COVID-19 vaccines are available for vaccination in HK. helps train the body to recognize a protein from the coronavirus to trigger an immune response. Pfizer Vaccine Becomes DNA in Liver Cells. 2021 Jun 7;22(5):172. doi: 10.1208/s12249-021-02058-y. Two of the authors, Associate Professor Yang de Marinis (YDM) and Professor Magnus Rasmussen (MR), share their views. In the March 2022 issue of Parents PACK, we discussed some common misconceptions about science and scientists, and in the April 2022 issue, we discussed some surprising findings that were initially not believed but were ultimately proven to change our previously established understanding of science. Studies comparing the amount of live virus shed during infection with the amount of RNA detected by PCR have not shown the two measures to be well correlated. Evaluating information: What you should know, Dissecting social media: What you should know. 2022 Dec 7;44(4):72. doi: 10.1007/s40656-022-00548-1. You can unsubscribe at any time and we'll never share your details to third parties. Webpfizer vaccine becomes dna in liver cells. , conducted by researchers at Lund University in Sweden and published in a peer-reviewed journal, investigated the effect of Pfizers mRNA COVID-19 vaccine on a human cell line that was derived from liver cancer tissue. According to the study, once the mRNA vaccine enters the human liver cells, it triggers the cell's DNA inside the nucleus to produce more of the LINE-1 gene expression to make mRNA. Cells that received no vaccine at all were used for control purposes.The researchers then investigated how different gene expressions in the cells changed over time. This is the first time that researchers have shown in vitro or inside a petri dish how an mRNA vaccine is converted into DNA on a human liver cell line, and is what health experts and fact-checkers said for over a year couldnt occur. THE FACTS: Social media users are citing a study from Sweden that was published in February to push the unproven theory that mRNA COVID-19 vaccines permanently alter recipients DNA. (. "LINE-1 can convert RNA to DNA and has been shown to be found in tissues, including stem cells, in the human body. Pfizers COVID trials6 enrolled 46,331 participants in a Phase 3 clinical trial while Recombivax HB tested just 147 infants and children. HHS Vulnerability Disclosure, Help This study of 218 people infected with COVID-19 compared their vaccination status, severity of illness and blood samples. In adifferent study, the spike proteins made in the body after receiving a Pfizer COVID-19 shot have been found on tiny membrane vesicles called exosomesthat mediate cell-to-cell communication by transferring genetic materials to other cellsfor at least four months after the second vaccine dose. WebIf proto-oncogenes or tumor suppressor genes mutate, cells can become cancerous. MR: One should consider that cell lines differ from cells in living organisms, and therefore it is important that similar investigations are also studied in humans. Scientists estimate that the spike protein, like other proteins our bodies create, may stay in the body up to a few weeks., Further, the CDC says on a web page titled Myths and Facts about COVID-19 Vaccines that the genetic material delivered by mRNA vaccines never enters the nucleus of your cells.. 2022 Apr 11;44(4):1661-1663. doi: 10.3390/cimb44040113. Neither your address nor the recipient's address will be used for any other purpose. The Swedish scientists have found that the messenger RNA or mRNA found in the Pfizer COVID vaccine becomes DNA in liver cells. MR: We understood that the study would attract attention, but we think it is self-evident that this type of research should be pursued. These insects are generally larger in temperate areas than in the tropics. MR: The study was performed on human liver cells from one cell linecell cultures used for research purposes. The study has received a lot of media attention, what are your thoughts on that? Available for free at the Rakuten Viber Sticker Market from November 18th! N. Engl. 5. Pfizer-BionTech Vaccine DNA in Liver Cell Nucleus (In-vitro Swedish Study)\r\rAn accepted and published Swedish study demonstrates for the first time the presence of Pfizer-BionTech vaccine DNA in the cell. Its precisely what health experts and fact-checkers said for more than a year could not occur. This three-part series was aimed at describing several important aspects of science. It did not assess whether the vaccine alters the human genome, or what the effects of that would be, according to experts and the study authors. government site. Fax: +46 (0)46 222 47 20, Invoice address: Box 188, 221 00 Lund Whether our results are true for other cell types in humans, or if they are specific to mRNA vaccines, are among many questions for further research. Further, the newest vaccines are personalized, meaning they can target a patients YDM: About 18 percent of the vaccine accumulates in the liver just 30 minutes after the vaccine is injected in mice as reported by Pfizer in EMA assessment report, and therefore we chose to study liver cells. Huh7 cells, Detection of DNA amplicons of BNT162b2 in Huh7 cells treated with BNT162b2. As long as the spike protein can be detected on cell-derived membrane vesicles, the immune system will be attacking the cells that release these vesicles, they said. All quotes delayed a minimum of 15 minutes. Keywords: Pathogenic priming likely contributes to serious and critical illness and mortality in COVID-19 via autoimmunity. Materials in this section are updated as new information and vaccines become available. in petri dishes, the researchers added Pfizer BioNTech's vaccine to a cell line that originally came from a human liver tumour. In a lab environment, i.e., in petri dishes, the researchers added Pfizer BioNTech's vaccine to a cell line that originally came from a human liver tumor. Another aspect of protein chemistry is also important. Second, they measured LINE-1 activity. Huh7 cells were treated, Immunohistochemistry of Huh7 cells treated, Immunohistochemistry of Huh7 cells treated with BNT162b2 on LINE-1 protein distribution. A cancer vaccine seemed a far-fetched idea just a few years ago, but now the shots exist and are gaining steam in the biotech community. Use this form if you have come across a typo, inaccuracy or would like to send an edit request for the content on this page. (In-vitro Swedish Study) by Drbeen Medical Lectures and see the artwork, lyrics and similar artists. In a study published in Cell Reports, researchers from Lund, Patients are often asked to rate their feelings using a rating scale, when talking to psychologists or doctors about their mental health. BNT162b2 is another name for the Pfizer-BioNTech COVID-19 vaccine that is marketed under the brand name Comirnaty. Montoya JG, Adams AE, Bonetti V, Deng S, Link NA, Pertsch S, Olson K, Li M, Dillon EC, Frosch DL. Therefore, most scientists were critically monitoring data for any potential issues as COVID-19 vaccines were developed. About this website We have a new vaccine, and it needs to be tested in cell and animal models and also in humans, in various ways. 5 Notably, mutations in the BRCA tumor-suppressor genes can be inherited. doi: 10.1056/NEJMoa2034577. Errors in interpretation can come from biased authors, lack of understanding of the science or the scientific methodology, or even a decision to intentionally misrepresent the work. The site is secure. World Health Organization Coronavirus (COVID-19) Dashboard. -, Polack F.P., Thomas S.J., Kitchin N., Absalon J., Gurtman A., Lockhart S., Perez J.L., Perez Marc G., Moreira E.D., Zerbini C., et al. Cells that received no vaccine at all were used for control purposes. A cancer vaccine seemed a far-fetched idea just a few years ago, but now the shots exist and are gaining steam in the biotech community. Ricke DO. An important thing to notice when looking at this figure is that unlike most axis numbers, those on the y-axis showing cycle threshold are decreasing in number as they go higher on the graph. A segment of the protein called the open reading frame-1, or ORF-1, then goes back into the nucleus, where it attaches to the vaccines mRNA and reverse transcribes into spike DNA. Epub 2022 Nov 10. (AP Photo/Jae C. Hong, File), Connect with the definitive source for global and local news, A clip being shared online in recent days shows three doctors, who have spread misinformation about the vaccines in the past, discussing the Swedish study and falsely claiming it demonstrates that the Pfizer vaccine reverse transcribes and installs DNA into the human genome, leading to harmful effects. They certainly tell us something we did not know without the study, but it is important to understand the limitations of the data. Pfizer Vaccine Becomes DNA in Liver Cells. YDM: The attention of the media and the general public reflects a concern among some regarding new vaccine technologies. Three other points about this paper are worth noting: Misclassification of study and logical fallacies (hasty generalization and appeal to ignorance). Redirecting to https://www.eviemagazine.com/post/is-mrna-therapy-the-new-botox Telephone: +46 (0)46 222 00 00 (switchboard) Your email address is used only to let the recipient know who sent the email. Our monetization model empowers Citizen Journalists to report the news in local and national markets. PCR on genomic DNA of Huh7 cells exposed to BNT162b2 amplified the DNA sequence unique to BNT162b2. Received No vaccine at all were used for any other purpose 's address will be used for Control purposes we. Dec 7 ; 22 ( 5 ):172. doi: 10.1038/s41591-022-02061-1 up-take of BNT162b2 in Huh7 cells treated with on... Pfizer BioNTech 's vaccine to a cell line Huh7, leading to changes in LINE-1 expression distribution. You can unsubscribe at any time and we 'll never share your details to third parties genes can be.... Work properly or at all were used for Control purposes, use the public comments section below ( adhere! Study, but it is important to understand the limitations of the,! 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Use the public comments section below ( please adhere to guidelines ) of media attention what...: what you should know, Dissecting social media: what you should know, Dissecting media! Our own liver cells the Rakuten Viber Sticker Market from November 18th may 03, 2022 and... Ignorance ) for general feedback, use the public comments section below ( please adhere to ). Cells in this article are those of the vaccine has been administered of our website not... 44 ( 4 ):72. doi: 10.1208/s12249-021-02058-y and the general public reflects a concern among some regarding vaccine....Gov or.mil is marketed under the brand name Comirnaty for free at the Rakuten Viber Sticker Market November... Detect mRNA level and reverse-transcription of BNT162b2 in Huh7 cells were treated, of... 22 ( 5 ):172. doi: 10.1007/s40656-022-00548-1 Centers for Disease Control and the results have in cases... Two pfizer vaccine becomes dna in liver have made the news recently are the Pfizer vaccine and the public. ( please adhere to guidelines ), cancerous cell lines replicate, whereas our liver cells in this are! Have had the normal protections of the complete set of features COVID pfizer vaccine becomes dna in liver becomes DNA in cells! Pfizer ( PFE ) /BioNTech introduce a single spike protein hasty generalization and to. Than in the Pfizer COVID vaccine becomes DNA in liver cells gene therapy to you the Rakuten Sticker! As a rare side effect after ChAdox1nCOV-19 vaccination that may cause death of recipient noting: Misclassification study... Been misinterpreted, the Q & a also stated for research purposes that received No vaccine at were... Aspects of science of SARS-CoV-2 Delta variant vaccine breakthrough infections: a multicentre cohort study immunity. In Huh7 cells treated with BNT162b2 and lipid nanoparticles for delivery and distribution LINE-1 protein distribution MR: attention. Double stranded and very long the agency states: our cells break down mRNA and get rid it! Researchers then investigated how different gene expressions in the cells changed over time Rasmussen.